GenNext Technolgies
Hydroxyl Radical Protein Footprinting (HRPF) can be analyzed using FoxWare Data Processing Software to map binding interactions between proteins and antibodies. Changes in residue-level oxidation of TNFα between bound (to Adalimumab) and unbound states are calculated based on XIC-level differences validated via 100-400 PSMs per individual peptide. New innovations in the software tool allow automation of residue-level changes with confidence metrics that inform upon spectral quality.
