This webcast will present recent studies of membrane proteins applying mass photometry – a relatively new technology that measures the mass of single proteins and complexes using light. Mass photometry is proving useful in studies of protein complexes – in solution as well as on membranes. Among the advantages of mass photometry is the small volume and low concentration of protein sample needed for analysis (1 µL at 200 nM concentration). This small amount is relevant for eukaryotic membrane proteins, which are difficult to purify in large amounts. The speakers will present work studying two examples of macromolecular complexes formed by eukaryotic membrane proteins. In both cases, mass photometry was crucial to addressing highly relevant physiological questions. They will explain what mass photometry can do, and what makes it suitable for studies of membrane protein complexes.
